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PCMD COMPOUND BANK
The first Compound Bank of Pakistan was established at Dr. Panjwani Center for
Molecular Medicine and Drug Research to collect and organize the wealth
of natural and synthetic compounds which are isolated and synthesized at the
International Center for Chemical and Biological Sciences, and other collaborating
institutions.
  
  
Objectives:
The objectives of the PCMD Compound Bank are:
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To support the drug related research at the center
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To manage the in vitro/ in vivo assays for various biological activities
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To apply advanced software tools to organize the structural and biological data
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To organize and preserve the compounds library into a 96-well plate format for High Throughput Screening (HTS) and for future use.
The Classical Research Process Designed for High Throughput Screening
PCMD compounds library in 96- well plate format
LIQUIDATOR 96
Manually operated 96-channel pipetting device
Micro plate Centrifuge and Concentrator
The main activities of the Compound Bank include:
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Compounds are received from ICCBS and other institutions, along with sample submission forms
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Quantity, purity, and authenticity of samples are checked by physical parameters
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Compounds entry record is maintained into various categories, depending on source and structural types, and arranged for various types of biological screenings.
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Biological activity results are compiled, and communicated to the sample providers
Chemfinder Database
MOE Database
Bioassays
Following biological assays are routinely conducted by the Compound Bank of
PCMD.
(a) Enzyme Inhibition Studies by Biochemical Assays
- Phosphodiesterase Inhibition
- Urease Inhibition
- Thymidine Phosphorylase Inhibition
- Acetylecholinesterase Inhibition
- Butyrylcholinesterase Inhibition
- α- Glucosidase Inhibition
- Lipoxyginase Inhibition
- Chymotrypsin Inhibition
- β-Glucuronidase Inhibition
(b) Antioxidant Studies
- DPPH Radical Scavenging Assay
- ABTS Radical Scavenging Assay
- Superoxide Anion Scavenging Assay
- Nitric Oxide Scavenging Assay
- Xanthine Oxidase Inhibition Assay
- Fe Chelating Assay
(c) Bench-top Bioassays
- Antibacterial Activity
- Antifungal Activity
- Antileishmanial Activity
- Insecticidal Activity
- Phytotoxic Activity
- Brine-Shrimp Lethality Assay
- Antimalarial Activity
(d) Antiglycation (Proteins) Studies
(e) Immunomodulatory/ Cytotoxicity Assays
(f) Antileishmanial Assays against Leishmania major
(g) Antimalarial Assays against Plasmodium falciparum
(h) Cytotoxicity Assays against normal cell lines
(i) Anticancer Assays against a range of cancer cell lines
Download Sample submission form
For Information
Please contact:
Ms. Sadia Siddiq
Manager,
PCMD Compound Bank
Telephone: +9221 4824930/ 4824934/4824936 Ext: 323
E-mail: sadia@iccs.edu
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