NMR Spectroscopy tutorial
The study of the interaction of electromagnetic radiations with matter is called spectroscopy.Nuclear magnetic resonance, which is abbreviated as NMR, is the basic phenomenon of NMR technique. NMR Spectroscopy is the technique which takes advantage of the magnetic properties of certain nuclei.In principal, all the atoms which possess property of "Spin" experience this phenomenon.However, the most important applications in practice are Proton NMR and C-13 NMR. When an NMR active nucleus is placed in a magnetic field, it absorbs energy at a frequency characteristic of the isotope. The intensity of the signal, absorption of the energy and resonant frequency are proportional to the strength of magnetic field. For example in a 7.05 tesla magnetic field, proton (1H) resonates at 300 MHz.
With the help of this tool, we can study physical, chemical and biological properties of matter. Chemists regularly use NMR Spectroscopy to study chemical structure of molecules. NMR Spectroscopy is a non-destructive tool through which good data can be obtained from samples weighing less than a milligram. It is the only technique for which a complete analysis and interpretation of the entire spectrum is normally expected. Molecules containing NMR active nuclei in different positions of the structure produce patterns of resonant frequencies. Analysis of this frequency spectrum determines the complete structure of the molecule.